Journal: Journal of Nanobiotechnology

Article Title: Neutrophil membrane-coated and MMP2-responsive nanoparticles deliver PIM1 inhibitor to alleviate inflammatory arthritis through inhibiting Th17 cell differentiation

doi: 10.1186/s12951-026-04280-x

Figure Lengend Snippet: NM@MRP-NP inhibits Th17 cell differentiation in an MMP2-responsive manner. ( A ) Flow cytometric analysis of the proportions of Th17 cells treated with vehicle, free AZD1208, MRP-NP, NM@MRP-NP, MMP2 or NM@MRP-NP+MMP2 ( n = 9). ( B ) Western blotting analysis of the expression of RORγt and pSTAT3 in cells treated with vehicle, free AZD1208, MRP-NP, NM@MRP-NP, MMP2 or NM@MRP-NP+MMP2 ( n = 9). ( C ) RT‒qPCR analysis of the expression of IL-17 A, IL-17 F, IL-26, CXCR6, and GM-CSF in cells treated with vehicle, free AZD1208, MRP-NP, NM@MRP-NP, MMP2 or NM@MRP-NP+MMP2 ( n = 9). The statistical analyses were performed as follows: one-way ANOVA followed by Bonferroni’s post hoc comparisons test ( A - C ). *** indicates p < 0.001, **** indicates p < 0.0001, and n.s. indicates no statistical significance

Article Snippet: Mouse TNF-α ELISA Kit (Elabscience, #E-EL-M3063) and mouse GM-CSF ELISA Kit (Elabscience, #E-EL-M0032) were used.

Techniques: Cell Differentiation, Western Blot, Expressing

Journal: Journal of Nanobiotechnology

Article Title: Neutrophil membrane-coated and MMP2-responsive nanoparticles deliver PIM1 inhibitor to alleviate inflammatory arthritis through inhibiting Th17 cell differentiation

doi: 10.1186/s12951-026-04280-x

Figure Lengend Snippet: PIM1 promotes Th17 cell differentiation by enhancing the activity of KGDH, PDH and F₀F₁-ATPase. ( A ) Flow cytometric analysis of the proportion of Th17 cells treated with OLI or CPI in the presence or absence of PIM1 overexpression ( n = 9). ( B ) Western blotting analysis of the expression of RORγt and pSTAT3 in cells treated with OLI or CPI in the presence or absence of PIM1 overexpression ( n = 9). ( C ) RT‒qPCR analysis of the expression of IL-17 A, IL-17 F, IL-26, CXCR6, and GM-CSF in cells treated with OLI or CPI in the presence or absence of PIM1 overexpression ( n = 9). The statistical analyses were performed as follows: one-way ANOVA followed by Bonferroni’s post hoc comparisons test ( A - C ). **** indicates p < 0.0001, and n.s. indicates no statistical significance

Article Snippet: Mouse TNF-α ELISA Kit (Elabscience, #E-EL-M3063) and mouse GM-CSF ELISA Kit (Elabscience, #E-EL-M0032) were used.

Techniques: Cell Differentiation, Activity Assay, Over Expression, Western Blot, Expressing

Journal: Journal of Nanobiotechnology

Article Title: Neutrophil membrane-coated and MMP2-responsive nanoparticles deliver PIM1 inhibitor to alleviate inflammatory arthritis through inhibiting Th17 cell differentiation

doi: 10.1186/s12951-026-04280-x

Figure Lengend Snippet: NM@MRP-NP inhibites Th17 cell response in the inflamed ankles. ( A ) Immunofluorescence analysis of CD4+RORγt+ cells in the ankles of SKG mice treated with vehicle, free AZD1208, MRP-NP or NM@MRP-NP. Scale bar = 50 μm. ( B ) Flow cytometric analysis of the proportion of Th17 cell in ankle joint tissues of SKG mice treated with vehicle, free AZD1208, MRP-NP or NM@MRP-NP ( n = 5). ( C ) Immunohistochemical analysis of IL-17 A+ cells in the ankles of SKG mice treated with vehicle, free AZD1208, MRP-NP or NM@MRP-NP. Scale bar = 100 μm. ( D ) RT‒qPCR analysis of the expression of RORγt and IL-17 A in the ankles of SKG mice treated with vehicle, free AZD1208, MRP-NP or NM@MRP-NP ( n = 5). ( E ) ELISA analysis of the concentration of TNF-α and GM-CSF in synovial fluid of SKG mice treated with vehicle, free AZD1208, MRP-NP or NM@MRP-NP ( n = 5). The statistical analyses were performed as follows: one-way ANOVA followed by Bonferroni’s post hoc comparisons test ( B - D ). * indicates p < 0.05, ** indicates p < 0.01 and *** indicates p < 0.001

Article Snippet: Mouse TNF-α ELISA Kit (Elabscience, #E-EL-M3063) and mouse GM-CSF ELISA Kit (Elabscience, #E-EL-M0032) were used.

Techniques: Immunofluorescence, Immunohistochemical staining, Expressing, Enzyme-linked Immunosorbent Assay, Concentration Assay

Journal: Journal of Nanobiotechnology

Article Title: Neutrophil membrane-coated and MMP2-responsive nanoparticles deliver PIM1 inhibitor to alleviate inflammatory arthritis through inhibiting Th17 cell differentiation

doi: 10.1186/s12951-026-04280-x

Figure Lengend Snippet: NM@MRP-NP inhibites Th17 cell response in the inflamed ankles. ( A ) Immunofluorescence analysis of CD4+RORγt+ cells in the ankles of SKG mice treated with vehicle, free AZD1208, MRP-NP or NM@MRP-NP. Scale bar = 50 μm. ( B ) Flow cytometric analysis of the proportion of Th17 cell in ankle joint tissues of SKG mice treated with vehicle, free AZD1208, MRP-NP or NM@MRP-NP ( n = 5). ( C ) Immunohistochemical analysis of IL-17 A+ cells in the ankles of SKG mice treated with vehicle, free AZD1208, MRP-NP or NM@MRP-NP. Scale bar = 100 μm. ( D ) RT‒qPCR analysis of the expression of RORγt and IL-17 A in the ankles of SKG mice treated with vehicle, free AZD1208, MRP-NP or NM@MRP-NP ( n = 5). ( E ) ELISA analysis of the concentration of TNF-α and GM-CSF in synovial fluid of SKG mice treated with vehicle, free AZD1208, MRP-NP or NM@MRP-NP ( n = 5). The statistical analyses were performed as follows: one-way ANOVA followed by Bonferroni’s post hoc comparisons test ( B - D ). * indicates p < 0.05, ** indicates p < 0.01 and *** indicates p < 0.001

Article Snippet: Mouse TNF-α ELISA Kit (Elabscience, #E-EL-M3063) and mouse GM-CSF ELISA Kit (Elabscience, #E-EL-M0032) were used.

Techniques: Immunofluorescence, Immunohistochemical staining, Expressing, Enzyme-linked Immunosorbent Assay, Concentration Assay